human intestinal epithelial cell lines ls174t  (ATCC)

Journal: Biochemical pharmacology

Article Title: Tryptophan 299 is a conserved residue of human pregnane X receptor critical for the functional consequence of ligand binding

doi: 10.1016/j.bcp.2016.02.009

Figure Lengend Snippet: LS174T cells transiently transfected with empty vector (EV), WT hPXR (hPXR), or hPXR mutant as indicated, CYP3A4-luc, and CMV-Renilla were treated with DMSO or different concentrations of agonists as indicated for 24 h prior to luciferase assay.

Article Snippet: 2.1 Materials HepG2 human liver carcinoma cells and LS174T human intestinal epithelial cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Transfection, Plasmid Preparation, Mutagenesis, Luciferase